diff-quik solution kit Search Results


86
Sysmex Corporation diff quik solution kit
Diff Quik Solution Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Medion Diagnostics diffquick kit solutions
Diffquick Kit Solutions, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Medion Diagnostics diff quick staining solution kit
Diff Quick Staining Solution Kit, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson diff-quik stain kit
Diff Quik Stain Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Polysciences inc diff-quik staining
Diff Quik Staining, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seikagaku corporation cell proliferation assay kit tetracolor one
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
Cell Proliferation Assay Kit Tetracolor One, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher purelink rna mini kit
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
Purelink Rna Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM 0.05 % toluidine blue solution (ph 4.1)
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
0.05 % Toluidine Blue Solution (Ph 4.1), supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss 700 lsm confocal microscope
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
700 Lsm Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson matrigel basement membrane matrix
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
Matrigel Basement Membrane Matrix, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences matrigel invasion chamber transwell polycarbonate membrane inserts
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
Matrigel Invasion Chamber Transwell Polycarbonate Membrane Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega celltiter-glo luminescence cell viability assay kit
Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited <t>proliferation</t> of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by <t>TetraColor</t> One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.
Celltiter Glo Luminescence Cell Viability Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited proliferation of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by TetraColor One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.

Journal: PLoS ONE

Article Title: Anti-Tumor Activity of a Novel HS-Mimetic-Vascular Endothelial Growth Factor Binding Small Molecule

doi: 10.1371/journal.pone.0039444

Figure Lengend Snippet: Cells were seeded in ACEA's 96× e-plate™ at a density of 5×10 3 cells per well, and continuously monitored using the RT-CES system up to 24 h, at which point Compound 8 (2 to 50 µM) was added. The cell index is plotted against time. The arrow indicates the time of the addition of compound 8 . Data represent the mean values ± S.D. for three identical wells from three independent experiments. B , compound 8 poorly inhibited proliferation of UV♀2 under serum-replete conditions (FBS), but potently inhibited VEGF-dependent proliferation of UV♀2 cells as analysed by TetraColor One assay. Results were normalised to DMSO controls. Data represent mean values ± SD for three independent experiments. * P <0.05 versus control. ** P <0.01 versus control. C , compound 8 inhibits VEGF-stimulated proliferation of human vascular endothelial cells. HUVEC cells were seeded on 6-well plates at a density of approximately 1×10 5 cells/well in M200 medium supplement with LSGS (Low serum growth Supplement). The next day cells were stimulated with 10 ng/mL of VEGF in the presence or absence of 1 µM and 10 µM compound 8 . After 48 hrs, Alamar Blue was added directly into culture media at a final concentration of 10% and the plate was returned to the incubator. Optical density (OD) of the plate was measured at 540 and 630 nm. As a negative control, AB was added to medium without cells.

Article Snippet: The cell proliferation assay kit Tetracolor One was obtained from Seikagaku Corp. (Tokyo, Japan) and the Diff-Quick solution was from International Reagent Corp. (Kobe, Japan).

Techniques: Control, Concentration Assay, Negative Control